Increased Sensitivity of One-Tube, Quantitative RT-PCR
نویسندگان
چکیده
منابع مشابه
Increased sensitivity of one-tube, quantitative RT-PCR.
With the introduction of real-time, fluorescence-based 5′ nuclease PCR (3,4) and instruments such as the ABI PRISM 7700 (TaqMan) sequence detector (Applied Biosystems, Foster City, CA, USA), quantitative RT-PCR is now a widely accepted method for measuring gene expression levels. Quantitative RTPCR is a sensitive technique and is particularly useful for the analysis of samples containing limi...
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Something I notice often in supervising new graduate students is their frustration with the irreproducibility of their biological data. For example, I am asked frequently ‘‘how many biological replicates do I need for qRT-PCR’’? The answer, of course, lies first with the students themselves. They need as many replicates as will persuade them of the validity of their observations. However, for m...
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Reverse transcription followed by quantitative polymerase chain reaction analysis, or qRT-PCR, is an extremely sensitive, cost-effective method for quantifying gene transcripts from plant cells. The availability of nonspecific double-stranded DNA (dsDNA) binding fluorophors, such as SYBR Green, and 384-well-plate real-time PCR machines that can measure fluorescence at the end of each PCR cycle ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00294bm06